A messenger molecule governs interdependency in an evolved Pseudomonas aeruginosa biofilm community

Biofilm populations are known to harbor great diversity, but the importance of this diversity is not fully understood. A likely contributor to this variation is the second messenger molecule cyclic-di-GMP: low levels associate with a planktonic lifestyle while high levels favor biofilm formation. In ongoing studies of an evolving biofilm population of Pseudomonas aeruginosa (PA), we observed extensive ecological diversification that may relate to this lifestyle switch. In PA, the gene bifA encodes a phosphodiesterase that is known to degrade cyclic-di-GMP and reduce biofilm. This gene was cloned onto a plasmid under control of an inducible promoter and the plasmid was added to each of seven different members of a diverse biofilm community. We also constructed communities lacking a single member. The fitness of each biofilm community, with a single member expressing bifA, lacking a member, or unaltered, was then quantified. Reductions in community fitness were observed when a single genotype expressed bifA and hence experienced low levels of cyclic-di-GMP. This result demonstrates that this messenger molecule regulates the biofilm lifestyle at the population level as well as the individual level. Subtraction of individual community members produced varied effects on fitness that associated with their ecological role. Together these results suggest that the synergy of diverse biofilms depends on a balance of genotypes varying in their regulation of molecules that define their niches

The Correlation of Dental Arch Width and Ethnicity

This study sought to demonstrate a correlation between arch width, ethnic background, individual height, weight, and whether orthodontic treatment had been rendered. Conclusions revealed that arch widths were significantly larger (p= 0.002 for the mandible and p= 0.008 for the maxilla) in non-Whites than in Whites. In addition, arch widths of the mandible were significantly larger in individuals who had had orthodontic treatment compared to those who had not (p=0.005). This did not carry through to those arch widths in the maxilla of orthodontic versus nonorthodontic care (p=0.258)

Microtransformers: controlled microscale navigation with flexible robots

Artificial microswimmers are a new technology with promising microfluidics and biomedical applications, such as directed cargo transport, microscale assembly, and targeted drug delivery. A fundamental barrier to realising this potential is the ability to control the trajectories of multiple individuals within a large group. A promising navigation mechanism for "fuel-based" microswimmers, for example autophoretic Janus particles, entails modulating the local environment to guide the swimmer, for instance by etching grooves in microchannels. However, such techniques are currently limited to bulk guidance. This paper will argue that by manufacturing microswimmers from phoretic filaments of flexible shape-memory polymer, elastic transformations can modulate swimming behaviour, allowing precision navigation of selected individuals within a group through complex environments

The Quantification of Tooth Displacement

By using reference points from a single pixel marker placed at the center point of the cuspid teeth and the center point on each of the incisor teeth, a polynomial curve was generated as a native curve for each dental arch studied. The polynomial curve generated from actual tooth position in each arch provides the forensic odontologist with another reference point that is quantifiable. The study represents that individual characteristics, such as tooth displacement, can be quantified in a simple, reliable, and repeatable format

Diagnosis and treatment of hereditary hemorrhagic telangiectasia in a pediatric patient with chronic cyanosis.

Background: Hereditary hemorrhagic telangiectasia (HHT) is an autosomal dominant disorder of vascular dysplasias that is characterized by mucocutaneous telangiectasias, gastrointestinal tract bleeding, and arteriovenous malformations (AVMs) of the pulmonary, hepatic, and cerebral vascular systems. Case Summary: A seven-year-old boy presented to his primary care provider with a five-day history of watery diarrhea and was incidentally found to have oxygen saturations in the low eighties, perioral cyanosis, and clubbing on physical exam. He was referred to the pediatric emergency department (ED) for evaluation. Upon presentation to the pediatric ED, oxygen saturation ranged from 74-85%. He was in no acute distress and was afebrile with normal vital signs. The patient was small for his age with height and weight in the thirteenth and third percentiles, respectively, and had central cyanosis and clubbing of fingers and toes. Auscultation revealed diminished lung sounds in the right lower lobe. No mucocutaneous telangiectasias or cardiac murmurs were appreciated. Family history was positive for hereditary hemorrhagic telangiectasia (HHT) with gastrointestinal bleeding and anemia in his maternal great grandmother and maternal grandmother, minor bleeding and mucocutaneous telangiectasias in his mother, and cyanosis with exercise and recurrent epistaxis in his brother. Complete blood count was significant for a hemoglobin level of 18.1 g/dL. A chest x-ray (Figure 1) showed an airspace opacification within the superior segment of the right lower lobe suspicious for an arteriovenous malformation (AVM).peer-reviewe

Bayesian Inference for PCFGs via Markov Chain Monte Carlo

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